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iqtm 5 optical module real-time pcr detection system  (Bio-Rad)


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    Structured Review

    Bio-Rad iqtm 5 optical module real-time pcr detection system
    Iqtm 5 Optical Module Real Time Pcr Detection System, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/iqtm+5+optical+system/cfx96+touch+real+time+pcr+detection+system/pmc11077765-63-6-14
    Average 90 stars, based on 1 article reviews
    iqtm 5 optical module real-time pcr detection system - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Real-time Polymerase Chain Reaction:

    Article Title: Aquaporin-3 is involved in NLRP3-inflammasome activation contributing to the setting of inflammatory response
    Article Snippet: Reverse transcription was performed using an iScript TM cDNA Synthesis Kit (BioRad). .. Quantitative PCR (qPCR) was performed using an iQTM 5 Optical System (BioRad) with an SYBR Green mix (Takara) and specific pre-designed primers for AQP3 (Hs_AQP1_1_SG QT00212996), AQP6. (Hs_AQP6_1_SG QT00010633), AQP8 ( Hs_AQP8_1_SG QT00039123), AQP9. (Hs_AQP9_1_SG QT00017710), AQP11 ( Hs_AQP11_1_SG QT00230636), AQP12. (Hs_AQP12A_1_SG QT00236432), HPRT-1 (Hs_HPRT-1_1_SG QT00059066), IL6. (Hs_IL6_1_SG QT00083720), IL1B (Hs_IL1B_1_SG QT00021385), TNFA. (Hs_TNF_1_SG QT00029162) and NLRP3 (Hs_NLRP3_1_SG QT00029771) (Qiagen). .. THP-1 were washed twice with PBS before total RNA purification using the RNeasy mini kit (Qiagen) and treated with RNase-free DNase I (Invitrogen) for 30 min, according to the manufacturer’s recommendations.

    Article Title: Aquaporin-3 is involved in NLRP3-inflammasome activation contributing to the setting of inflammatory response.
    Article Snippet: Inflammasomes are large immune multiprotein complexes that tightly regulate the production of the pro-inflammatory cytokines, being dependent on cell regulatory volume mechanisms.. Aquaporins (AQPs) are protein channels that facilitate the transport of water and glycerol (aquaglyceroporins) through membranes, essential for cell volume regulation.. Although these membrane proteins are highly expressed in monocytes and macrophages, their role in the inflammatory process is still unclear.

    SYBR Green Assay:

    Article Title: Aquaporin-3 is involved in NLRP3-inflammasome activation contributing to the setting of inflammatory response
    Article Snippet: Reverse transcription was performed using an iScript TM cDNA Synthesis Kit (BioRad). .. Quantitative PCR (qPCR) was performed using an iQTM 5 Optical System (BioRad) with an SYBR Green mix (Takara) and specific pre-designed primers for AQP3 (Hs_AQP1_1_SG QT00212996), AQP6. (Hs_AQP6_1_SG QT00010633), AQP8 ( Hs_AQP8_1_SG QT00039123), AQP9. (Hs_AQP9_1_SG QT00017710), AQP11 ( Hs_AQP11_1_SG QT00230636), AQP12. (Hs_AQP12A_1_SG QT00236432), HPRT-1 (Hs_HPRT-1_1_SG QT00059066), IL6. (Hs_IL6_1_SG QT00083720), IL1B (Hs_IL1B_1_SG QT00021385), TNFA. (Hs_TNF_1_SG QT00029162) and NLRP3 (Hs_NLRP3_1_SG QT00029771) (Qiagen). .. THP-1 were washed twice with PBS before total RNA purification using the RNeasy mini kit (Qiagen) and treated with RNase-free DNase I (Invitrogen) for 30 min, according to the manufacturer’s recommendations.

    Article Title: Aquaporin-3 is involved in NLRP3-inflammasome activation contributing to the setting of inflammatory response.
    Article Snippet: Inflammasomes are large immune multiprotein complexes that tightly regulate the production of the pro-inflammatory cytokines, being dependent on cell regulatory volume mechanisms.. Aquaporins (AQPs) are protein channels that facilitate the transport of water and glycerol (aquaglyceroporins) through membranes, essential for cell volume regulation.. Although these membrane proteins are highly expressed in monocytes and macrophages, their role in the inflammatory process is still unclear.



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